PCR EXTRACTION KIT
Features
Analytes: ORF1ab, N gene and Internal control
Internal Control, The whole process is monitored by internal control
Sensitivity : 200 copies/mL
Rapid Reaction Time, 52 minutes,with a rapid PCR system
High Accuracy, The product has been approved by the state drug administration and obtained NMPA and CE certification. The kit has been verified by 600+ clinical samples and used in more than 100 hospitals and Centers for Disease Control and Prevention
Production information
Working Principle
This kit is used for in vitro qualitative detection of novel coronavirus 2019-nCoV in respiratory specimens including oropharyngeal swab, sputum, bronchoalveolar lavage fluid and nasopharyngeal swab. Primer sets and FAM labeled probe are designed for specific detection of ORFlab gene of 2019-nCoV, VIC labeled probe for N gene of 2019-nCoV. Human RNase P gene extracted concurrently with the test sample provides an internal control to validate nucleic extraction procedure and reagent integrity. Probe targeting human RNase P gene is labeled with CY5.
Applicable Instruments
Real-Time PCR System: Molarray MA-6000, ABI 7500, ViiATM 7, QuantStudio 5, QuantStudio 6/7 pro, QuantStudio 6/7 flex, Agilent Mx3000P/3005P, Rotor GeneTM 6000/Q, Bio-Rad CFX96 TouchTM/iQTM 5, Hongshi SLAN-96S/96P,
Roche Light Cycler 480, Molarray MA-1630Q, AGS8830, AGS4800.
Workflow
Sample Treatment Zone, Extract RNA with viral Extraction Kit. Positive and Negative control can be used without extraction
Mix Preparation Zone, Prepare Negative + RNA sample + Positive control. Add RT-PCR reaction buffer and RT-PCR enzyme mix to PCR tube.
Sample Treatment Area, Mix, close cap of tubes tightly and centrifuge.
PCR Amplification Zone, Put the caped PCR tubes into PCR Machine for amplification.











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